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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two added splice variants had been described but thorough nucleotide sequence info is not really but available.

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Blocking peptides are peptides that bind specially into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.

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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is particular.

Goal Info Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive read more expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and is also degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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